The Kit de diagnostic moléculaire 2019-nCoV is a TaqMan probe real-time fluorescent PCR pour détecter qualitativement l'acide nucléique des gènes ORF1ab et N de 2019-nCoV in throat swab, saliva in patients with respiratory infection symptoms and close contacts.

In this kit, primers and probes of real-time fluorescent PCR technology are designed to the conserved and specific regions of the ORF1ab and N genes of 2019-nCoV, respectively. During PCR amplification, the probe binds to the template, and the 5'-end reporter group of the probe is cleaved by the Taq enzyme (5'→3' exonuclease activity), thereby moving away from the quenching group to generate a fluorescent signal. The real-time amplification curve is automatically plotted based on the detected fluorescence signal, and the sample Ct value is calculated. FAM and HEX fluorophores are labeled to ORF1ab gene and N gene probes in 2019-nCoV-PCR reaction solution. By using one test, qualitative detection of the above two genes of 2019-nCoV can be performed simultaneously.

Le kit est fourni avec un contrôle interne (IC), qui est un fragment de gène domestique humain, et est marqué avec un groupe fluorescent ROX.